ND processing

These workflows show preprocessing capabilities on whole dimension (many frames). They can be part of more complex workflows or used standalone.

Align frames

Correlates the image contents frame-to-frame and translates each frame based on the calculated [x, y] offset.

Source z-stack	Aligned z-stack

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Extended depth of focus (EDF)

Reduces a whole stack into a single focused image. It analyzes each frame and takes only portions of the image which are in-focus.

Source z-stack	EDF

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MaxIP, Average and Median of all frames

All mentioned operations reduce whole stack into a single image.

Source movie	Max IP

Average	Median

Try it in NIS-Express:

• MaxIP
• Average
• Median

Difference of subsequent frames

Select a previous frame with Select Frame and Subtract it from the current frame. In the images below there is a plant root growing.

Source image $I_i$	Source image $I_{i+1}$

Image $I_{i+1}-I_i$	GA3 Schematic

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Stitch multi-point into large image

	0	1
0
1

Stitched image

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