Import individual files

Use this workflow to convert many files in one run, where each input file produces its own ND2 output.

Example conversion:

file1.tiff -> file1.nd2
file2.tiff -> file2.nd2
file3.tiff -> file3.nd2

Unlike sequence import, files are not merged into one ND2.

Step 1: Open conversion and choose files

• From any page, click the arrow on the Image button.
• Select Convert images....
• Choose one file from the target folder.

Page 1 lets you define which files will be converted:

• Selected file sets the starting point
• Pattern controls which files are included
• Dimensions mapping keeps interpretation consistent
• Output preview shows input -> output mapping
• Suffix adds custom text to output names

Step 2: Metadata input page

On this page you review and edit metadata for the batch conversion.

• Experiment settings let you define values like time interval, z-step, and channel-related metadata.
• Microscope settings let you define acquisition details used for output ND2 files.

After setting metadata, click Convert to start conversion.

Step 3: Conversion progress and results

On this page, you can monitor the current file being converted, overall progress (done / total), and the conversion log. If one file fails, the batch continues with the remaining files.

After conversion, a summary shows how many files were converted, failed, and processed in total.

Open ND2 file opens the last successfully converted file.