Import and convert images

Use this section to convert external image formats into ND2 so they can be processed in NIS-Express workflows.

Supported input formats: .nd2, .tif, .tiff, .btf, .lsm, .czi, .oif, .oib, .png, .jpg, .jpeg.

Single file conversion

  flowchart LR
    A[file.tiff] --> B[file.nd2]

Converts one input file into one ND2 output file.

Convert individual files

  flowchart LR
    subgraph In [Input Folder]
        direction TB
        A1[file1.tiff]
        A2[file2.tiff]
        A3[file3.tiff]
    end

    subgraph Out [Output Folder]
        direction TB
        B1[file1.nd2]
        B2[file2.nd2]
        B3[file3.nd2]
    end

    A1 --> B1
    A2 --> B2
    A3 --> B3

Converts each file independently.

Convert sequence to one ND2

  flowchart LR
    subgraph In [Input Folder]
        direction TB
        I1[file_t001_z001.tiff]
        I2[file_t001_z002.tiff]
        I3[file_t002_z001.tiff]
        I4[file_t002_z002.tiff]
    end

    O["sequence.nd2<br/>(t=2, z=2)"]

    I1 --> O
    I2 --> O
    I3 --> O
    I4 --> O

Combines multiple files that represent one dimensional sequence into a single ND2.

Import labeled sequence

Import channels with labels/binaries and convert them to binary layers in GA3.