Object position features

All features support bot 2D and 3D object. In 2D there two coordinates whereas in 3D there are three.

Center

Center (in µm) of mass of the object where all pixels have the same weight.

Center Px

Uncalibrated center (in px) of mass of the object where all pixels have the same weight.

Center Abs

Absolute Center (in µm) of mass of the object where all pixels have the same weight. The values are transformed to the stage coordinates.

Centroid

Center (in µm) of mass of the object where all pixels are weighted by selected intensity channel.

Centroid Px

Uncalibrated Centroid (in px) of mass of the object where all pixels are weighted by selected intensity channel.

Centroid Abs

Absolute Centroid (in µm) of mass of the object where all are weighted by selected intensity channel. The values are transformed to the stage coordinates.

Geodesic Center

A Geodesic Center (in µm) is a point within the object, such that the sum of its distances to every other point in the object is minimal. For convex objects, it corresponds to the center of gravity. The algorithm used to calculate the geodesic center is approximate.

Geodesic Center Px

An uncalibrated Geodesic Center (in px) is a point within the object, such that the sum of its distances to every other point in the object is minimal. For convex objects, it corresponds to the center of gravity. The algorithm used to calculate the geodesic center is approximate.

Start

Coordinates (in µm) of the first pixel encountered when scanning the image from the origin (top-left) in left-to-right direction from to to bottom.

Start Px

Uncalibrated coordinates (in px) of the first pixel encountered when scanning the image from the origin (top-left) in left-to-right direction from to to bottom.

Bounds

Distances (in µm) of the left, right, top, and bottom edges of the object bounding box.

Bounds Px

Uncalibrated Distances (in px) of the left, right, top, and bottom edges of the object bounding box.

Bounds Abs

Absolute distances (in µm) of the left, right, top, and bottom edges of the object bounding rectangle within a motorized stage range. This feature can be measured only if a motorized stage is connected.