Metadata features

Stage Position [µm]

The stage X, Y, Z position in microns at the moment the frame was captured.

• Z Stage position for the current frame for 2D node.
• Z Stack home position for the current volume for 3D node.

Time [s]

Acquisition time of the current image in seconds. If the image is a part of an image sequence, the time is measured from the beginning of the sequence.

Bit Depth

Calibration [µm/px]

Pixel X, Y calibration in microns per pixel.

Channel Name

Name of the channel the node is connected to.

Channel Info

Information about all channels the node is connected to. Channel name (excitation wavelength in nm)

For example:

Hoechst (385 nm)
Alexa 488 (475 nm)

Channel Metadata

Selected information about the selected channel.

Available metadata:

• Channel: channel name
• OC: name of then optical configuration
• Excitation WL: excitation wavelength in nm
• Emission WL: emission wavelength in nm
• Modality: Brightfield, DIC, Phase contrast, Wide-field fluorescence, …
• Magnification: objective magnification
• Numerical aperture: numerical aperture of the objective
• Immersion RI: refraction index of the immersion
• Pinhole size: pinhole size in µm

Frame Center [µm]

The center X, Y of the frame/image in microns. Half of the Frame Size.

Frame Center Px

The center X, Y of the frame/image in pixels. Half of the Frame Size Px.

Frame Size [µm]

X, Y size of the frame/image in microns.

Frame Size Px

X, Y size of the frame/image in pixels.

Volume Center [µm]

The center X, Y, Z of the volume in microns. Half of the Volume Size.

Volume Center Px

The center X, Y, Z of the volume in pixels. Half of the Volume Size Px.

Volume Size [µm]

X, Y, Z size of the volume in microns.

Volume Size Px

X, Y, Z size of the volume in pixels.

Max Allowed Value

Maximum possible pixel value. Calculated from Bit Depth ($b$)

• $2^b-1$ for integers ($b<32$).
• $\infty$ for floats ($b=32$).

Min Allowed Value

Minimum possible pixel value. Calculated from Bit Depth ($b$)

• $0$ for integers ($b<32$).
• $-\infty$ for floats ($b=32$).

Custom Metadata

Reads custom metadata field. Custom Metadata are global per whole file.

Recorded Data

Reads recorded data field for the current frame or home frame for volume in 3D.

Predefined recorded data fields:

• Cam1 ExposureTime
• Cam1 Temperature
• Cam2 ExposureTime
• Cam2 Temperature
• Count Index
• Exposure Time
• Exposure Time (Channel 1, …, Channel N)
• PFS Offset
• PFS Status
• Plate Barcode
• Plate Index
• Plate Name
• Plate Position
• Region Index
• Slide Barcode
• Slide Index
• Slide Name
• Slide Position
• Ti2 ZDrive
• Time
• TimeLapse Index
• Well
• Well Column
• Well Index
• Well Row
• X Coord
• XY Site Index
• XY Site Name
• Y Coord
• Z Coord
• Z Fast Coord
• Z Main Coord
• ZStack Index

Filename

Returns the filename (e.g. “sample.nd2”)

Filepath

Returns the full absolute file path (e.g. “C:\images\sample.nd2”).

Z Step

Returns the Z-Step form the Z-stack experiment definition or N/A.

Time Loop Interval

Returns the interval from the time-lapse experiment definition or N/A.

Time Loop Duration

Returns the duration from the time-lapse experiment definition or N/A.

Author

Returns the Author field from the Custom Metadata. Filled by Smart Experiment acquisition.

Username

Return the name of the current user.

Assay Name

Returns the Assay Name field from the Custom Metadata. Filled by Smart Experiment acquisition.

Assay Settings

Returns the Assay Settings field from the Custom Metadata. Filled by Smart Experiment acquisition.

Date Created

Returns the Date Created field from the Custom Metadata. Filled by Smart Experiment acquisition.

Wellplate ID

Returns the Wellplate ID field from the Custom Metadata. Filled by Smart Experiment acquisition.

Wellplate Type

Returns the Wellplate Type field from the Custom Metadata. Filled by Smart Experiment acquisition.