Feature availability
A checkmark shows that a feature is available in the variant: Basic or Advanced. A module badge shows that the module (Ai or Decon) is required.
General functionality
| Feature | Basic | Advanced |
|---|---|---|
| Recipe page | ✓ | ✓ |
| Image page | ✓ | ✓ |
| Ai page | ✗ | Ai |
Recipe page toolbar
| Feature | Basic | Advanced |
|---|---|---|
| Home | ✓ | ✓ |
| Image | ✓ | ✓ |
| Edit | ✓ | ✓ |
| Deconv | ✗ | Decon |
| Denoise | ||
| Denoising | ✓ | ✓ |
| Denoise.ai | ✗ | ✓ |
| ND Denoise.ai | ✗ | ✓ |
| ND | ||
| Align frames | ✓ | ✓ |
| Extended depth of focus (EDF) | ✗ | ✓ |
| Maximum intensity projection (MaxIP) | ✓ | ✓ |
| Average of all frames | ✓ | ✓ |
| Median of all frames | ✓ | ✓ |
| Difference of subsequent frames | ✓ | ✓ |
| Stitch multi-point to large image | ✓ | ✓ |
| Measure | ||
| Object count | ✓ | ✓ |
| Object catalog | ✓ | ✓ |
| Time measurement (channels) | ✓ | ✓ |
| Time measurement (ROIs) | ✓ | ✓ |
| Measure cells | ✓ | ✓ |
| Measure moving objects | ✗ | ✓ |
| Tracking motility | ✗ | ✓ |
| Single particle tracking | ✗ | ✓ |
| Live-dead assay | ✓ | ✓ |
| 3D object count | ✓ | ✓ |
| 3D tracking | ✗ | ✓ |
| 3D cell measurement | ✗ | ✓ |
GA3 nodes
The following nodes are available in Advanced. They are grouped as in the GA3 node reference.
| Feature | Basic | Advanced |
|---|---|---|
| Image processing | ||
| Deconvolution | ||
| Deconvolution | ✗ | Decon |
| Fluo. Unmixing | ✗ | ✓ |
| ND processing & conversions | ||
| Stack reduction | ||
| EDF | ✗ | ✓ |
| Python scripting | ||
| Python | ✗ | ✓ |
| Segmentation | ||
| Special detections | ||
| Cellpose3 | ✗ | ✓ |
| Cellpose SAM | ✗ | ✓ |
| EfficientV2-UNet | ✗ | ✓ |
| InstanSeg | ✗ | ✓ |
| StarDist | ✗ | ✓ |
| Binary operations | ||
| Classification | ||
| Detection Classifier | ✗ | ✓ |
| Data manipulation | ||
| JavaScript | ||
| JS Create Column | ✗ | ✓ |
| JS Create Table | ✗ | ✓ |
| Results & graphs | ||
| Graphs | ||
| Matplotlib | ✗ | ✓ |
| Reports | ||
| HTML Report | ✗ | ✓ |
| NIS.ai | ||
| Preprocessing | ||
| Clarify.ai | ✗ | Decon |
| Restore.ai | ✗ | Decon |
| Denoise.ai | ✗ | ✓ |
| ND Denoise.ai | ✗ | ✓ |
| Transformations | ||
| Enhance.ai | ✗ | Ai |
| Convert.ai | ✗ | Ai |
| Segmentation | ||
| Cells Localization.ai | ✗ | ✓ |
| Segment.ai | ✗ | Ai |
| Segment Objects.ai | ✗ | Ai |
| Trained files | ||
| Select Trained File ai | ✗ | Ai |
| Measurement | ||
| Cells Presence.ai | ✗ | ✓ |
| Quality Estimate.ai | ✗ | ✓ |
| Evaluation | ||
| Segmentation Accuracy | ✗ | Ai |
| Object Segmentation Accuracy | ✗ | Ai |
| Tracking | ||
| Tracking | ||
| Track Particles | ✗ | ✓ |
| Tracks | ||
| MSD | ✗ | ✓ |
| Tracking Features | ||
| Feature Derivative | ✗ | ✓ |
| Motion Features | ✗ | ✓ |
| Track Features | ✗ | ✓ |
| Results | ||
| Export Tracking | ✗ | ✓ |
Examples
| Feature | Basic | Advanced |
|---|---|---|
| ND | ||
| Align frames | ✓ | ✓ |
| Extended depth of focus (EDF) | ✗ | ✓ |
| Maximum intensity projection (MaxIP) | ✓ | ✓ |
| Average of all frames | ✓ | ✓ |
| Median of all frames | ✓ | ✓ |
| Difference of subsequent frames | ✓ | ✓ |
| Stitch multi-point into large image | ✓ | ✓ |
| Deconvolution | ||
| 2D deconvolution | ✗ | Decon |
| Measure | ||
| Object counting | ||
| Object count on a single RGB image | ✓ | ✓ |
| Object catalog on a single RGB image | ✓ | ✓ |
| Object count on a fluorescence time-lapse | ✓ | ✓ |
| Object catalog on a fluorescence time-lapse | ✓ | ✓ |
| Time measurement | ||
| Measure single field show values per channel | ✓ | ✓ |
| Measure and show many ROIs on a selected channel | ✓ | ✓ |
| Cell measurement | ||
| Detect and measure cell features | ✓ | ✓ |
| Tracking | ||
| Measure moving cells (Threshold) | ✓ | ✓ |
| Measure moving cells (Cellpose SAM) | ✗ | ✓ |
| Measure sperm motility | ✗ | ✓ |
| Single particle tracking | ✗ | ✓ |
| Wellplate assays | ||
| Live/dead assay | ✓ | ✓ |
| 3D counting and tracking | ||
| Object count | ✓ | ✓ |
| Tracking | ✗ | ✓ |
| Cell Measurement and counting using Cellpose SAM segmentation | ✗ | ✓ |